J. Technology and Education, Vol.12, No.2, pp.47-53, 2005

ノート

ホウ酸を用いたクエルセチンの簡便なケイ光定量法に関する研究

 

芳野恭士*、持山由規子、杉山笑美子、小川健二郎

沼津工業高等専門学校　物質工学科（〒410-8501　沼津市大岡3600）

*k-yoshino@numazu-ct.ac.jp

 

Convenient Fluorometric Determination of Quercetin Using Boric Acid

 

Kyoji YOSHINO, Yukiko MOCHIYAMA, Emiko SUGIYAMA, Kenjiro OGAWA

Numazu College of Technology (3600 Ooka, Numazu, Shizuoka 410-8501, Japan)

 

(Received November 22, 2005; Accepted December 14, 2005)

 

Abstract

A fluorometric quantitative method for determining flavonols was developed that uses boric acid to detect the chemical structure R−CX−C−CO−C＝C−R’ (X=O, OH, OCH₃).  The total flavonol levels in the plasma, liver, and kidney of mice administered orally with quercetin were determined by this analytical method.  The maximum excitation wavelength of the fluorophore generated in the reaction with the flavonol, i.e. quercetin, and boric acid was 460 nm and the maximum emission wavelength was 506 nm.  The calibration curve for quercetin was linear between 5.0 mg/mL and 0.50 mg/mL.  Recoveries of quercetin from the homogenates of mouse liver and kidney were good, at 80 ~ 90%, however, recovery from the plasma was too high, at around 130%.  In the plasma, liver and kidney of mice administered orally with quercetin at doses of 100 and 200 mg/kg body weight, the concentration of total flavonols 60 min after the administrations was dose dependent.  These results suggest that this analytical method could evaluate the concentration of total flavonols in the plasma, liver, and kidney of mice administered orally with quercetin, detecting the sum of the concentrations of the original compound and its metabolites retaining the chemical structure R−CX−C−CO−C＝C−R’ (X=O, OH, OCH₃).

Keywords: Quercetin, Boric acid, Fluorometric analysis, Mouse