J. Technology and Education, Vol.14, No.2, pp.37-44, 2007
沼津工業高等専門学校 物質工学科（〒410-8501 沼津市大岡3600）
Preventive Effects of Various Antioxidants on Mouse Contact Dermatitis
Kyoji YOSHINO, Yuko MIYAUCHI
Numazu College of Technology (3600 Ooka, Numazu, Shizuoka 410-8501, Japan)
(Received July 30, 2007; Accepted September 4, 2007)
The preventive effects of 10 phenolic compounds and 9 non-phenolic antioxidants on mouse contact dermatitis were investigated using an animal disease model of oxazolone-induced mouse ear edema in this study. With percutaeous administration of phenolic compounds at the challenge at a dose of 0.13 mg/ear, the anti-contact dermatitis activities were significantly correlated with the Fe2+-chelating activities but not with the O2-・-scavenging activities. In particular, compounds having galloyl moieties in their chemical structures, such as (-)-epicatechin gallate, (-)-epigallocatechin gallate, and 1,2,3,4,6-pentagalloyl glucose, showed strong anti-contact dermatitis activities and Fe2+-chelating activities. Among non-phenolic antioxidants, iron-chelators, hydroxyl radical-scavengers, and a lipoxygenase and cyclooxygenase inhibitor showed significant anti-contact dermatitis activities in mice. Administration of iron (III) chloride inversely enhanced mouse contact dermatitis. The percutaeous administration of non-phenolic iron-chelators and (-)-epigallocatechin gallate also showed significant anti-inflammatory activities in a mouse model of arachidonic acid-induced ear edema. Therefore, the iron-chelating activities of some phenolic and non-phenolic compounds are thought to be extremely effective for the prevention of the inflammation caused by oxazolone-induced contact dermatitis in mice.
Keywords: Phenolic compound, Antioxidative activity, Mouse, Contact dermatitis