J. Technology and Education, Vol.14, No.2, pp.37-44, 2007
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Preventive Effects of Various Antioxidants on Mouse Contact Dermatitis
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Kyoji YOSHINO, Yuko MIYAUCHI
Numazu College of Technology (3600 Ooka, Numazu, Shizuoka 410-8501, Japan)
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(Received July 30, 2007; Accepted September 4, 2007)
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Abstract
The preventive effects of 10 phenolic compounds and 9 non-phenolic antioxidants on mouse contact dermatitis were investigated using an animal disease model of oxazolone-induced mouse ear edema in this study.@With percutaeous administration of phenolic compounds at the challenge at a dose of 0.13 mg/ear, the anti-contact dermatitis activities were significantly correlated with the Fe2+-chelating activities but not with the O2-E-scavenging activities.@In particular, compounds having galloyl moieties in their chemical structures, such as (-)-epicatechin gallate, (-)-epigallocatechin gallate, and 1,2,3,4,6-pentagalloyl glucose, showed strong anti-contact dermatitis activities and Fe2+-chelating activities.@Among non-phenolic antioxidants, iron-chelators, hydroxyl radical-scavengers, and a lipoxygenase and cyclooxygenase inhibitor showed significant anti-contact dermatitis activities in mice.@Administration of iron (III) chloride inversely enhanced mouse contact dermatitis.@The percutaeous administration of non-phenolic iron-chelators and (-)-epigallocatechin gallate also showed significant anti-inflammatory activities in a mouse model of arachidonic acid-induced ear edema.@Therefore, the iron-chelating activities of some phenolic and non-phenolic compounds are thought to be extremely effective for the prevention of the inflammation caused by oxazolone-induced contact dermatitis in mice.
Keywords: Phenolic compound, Antioxidative activity, Mouse, Contact dermatitis
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