J. Technology and Education, Vol.15, No.1, pp.37-40, 2008

研究論文

　

ミジンコ遊泳阻害試験法の簡素化に関する検討

　

清水涼子 ，吉村忠与志, 高山勝己^*

　

福井工業高等専門学校物質工学科（〒916-8507福井県鯖江市下司町）

*takayama@fukui-nct.ac.jp

　

　

Simplification of a toxicity test using Daphnia magna

　

Ryoko SHIMIZU, Tadayosi YOSHIMURA, and Katsumi TAKAYAMA^*

　

Department of Chemistry & Biology Engineering, Fukui National College of Technology

(Geshi-cho, Sabae-shi, Fukui 916-8507, Japan)

　

(Received May 26, 2008; Accepted June 10, 2008)

　

Abstract

The inhibition of the mobility of Daphnia magna (EC₅₀) was effectively used to estimate the toxicity value of chemical agents. However, the protocol for this method is quite complex due to the strict conditions required for the vessel and Daphnia magna using for the test. Therefore, we used a 6- or 96-well disposable plate instead of a glass vessel and simplified the analytical procedure. The EC₅₀ for potassium dichromic acid was estimated to range in concentration from 0.5 to 2.5 ppb. Although this EC₅₀ value disagrees with those (0.9 ? 2.0 ppm) estimated by the traditional method (JIS K0229), our simplified method could still be useful to evaluate whether or not chemical agents have toxicity.

Keywords: Daphnia magna, Inhibition of the mobility, Toxicity test, 24h-EC, multi-well plate