J. Technology and Education, Vol.16, No.1, pp.1-7, 2009
Preventive Effects of Polysaccharide Fractions Prepared from Hypsizigus marmoreus and Various Glucans on Mouse Contact Dermatitis
Kyoji YOSHINO, Koichi OZAWA, Tomoaki YONEYAMA, Masayuki TAKEGUCHI
Numazu College of Technology (3600 Ooka, Numazu, Shizuoka 410-8501, Japan)
(Received January 19, 2009; Accepted February 6, 2009)
The preventive effects of two polysaccharide fractions prepared from Hypsizigus marmoreus and various glucans on mouse contact dermatitis were investigated by an animal disease model of oxazolone-induced mouse ear edema in this study.@The yields of two fractions obtained, Fr.1 and Fr.2, were 65.0%(w/w) and 0.7%(w/w), respectively.@Fr.1 and Fr.2 contained 62.6%(w/w) of insoluble dietary fibers and 83.6%(w/w) of soluble polysaccharides, respectively.@The oral administration of these fractions at a dose of 250mg/kg body weight, as well as their percutaneous administration at a dose of 0.10mg/ear, suppressed mouse contact dermatitis. The commercial indigestible glucans, two ?-glucans (barley ?-glucan and curdlan) and an ?-glucan (pullulan), also suppressed mouse contact dermatitis in cases of oral administration at doses of 200 and/or 250mg/kg body weight and percutaneous administration at a dose of 0.10mg/ear.@Therefore, the major active components in two polysaccharide fractions prepared from H. marmoreus are thought to be insoluble and soluble polysaccharides that are indigestible in animal bodies.
Keywords: Polysaccharide, Glucan, Mushroom, Contact dermatitis