J. Technology and Education, Vol.16, No.2, pp.47-51, 2009

研究論文

動画画像解析を用いたミジンコ泳動阻害試験

　

高山勝己^*、平井恵子、長瀬駿、鍛田侑吾、清水涼子、吉村忠与志

福井工業高等専門学校物質工学科（〒916-8507福井県鯖江市下司町）

*takayama@fukui-nct.ac.jp

　

Development of an image analysis system for toxicity testing using Daphnia magna

　

Katsumi TAKAYAMA,* Keiko HIRAI, Shun NAGASE, Yugo KAJITA,

Ryoko SHIMIZU and Tadayosi YOSHIMURA

　

Department of Chemistry and Biology Engineering, Fukui National College of Technology

(Geshi-cho, Sabae-shi, Fukui 916-8507, Japan)

　

(Received October 7, 2009; Accepted October 16, 2009)

　

Abstract

An on-line monitoring system for the toxicity assessment of chemicals using Daphnia magna was studied. The behavior of Daphnia magna in micro well plates for organophosphorus pesticides (OPs) was observed through a web camera connected to a personal computer. The stock image data were analyzed using a laboratory image analysis program. Daphnia magna showed abnormal behavior, such as a rotational motion, in the presence of OPs. The motion of Daphnia magna in the localized area can be used as an index to determine toxicity to pesticides, which is recognized by our image analysis system. The present method is much more time-efficient than the conventional technique based on Japanese Industrial Standards (JIS).

　

Key words:　Organophosphorus pesticides, On-line monitoring, Daphnia magna, motion analysis