J. Technology and Education, Vol.16, No.2, pp.47-51, 2009
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Development of an image analysis system for toxicity testing using Daphnia magna
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Katsumi TAKAYAMA,* Keiko HIRAI, Shun NAGASE, Yugo KAJITA,
Ryoko SHIMIZU and Tadayosi YOSHIMURA
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Department of Chemistry and Biology Engineering, Fukui National College of Technology
(Geshi-cho, Sabae-shi, Fukui 916-8507, Japan)
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(Received October 7, 2009; Accepted October 16, 2009)
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Abstract
An on-line monitoring system for the toxicity assessment of chemicals using Daphnia magna was studied. The behavior of Daphnia magna in micro well plates for organophosphorus pesticides (OPs) was observed through a web camera connected to a personal computer. The stock image data were analyzed using a laboratory image analysis program. Daphnia magna showed abnormal behavior, such as a rotational motion, in the presence of OPs. The motion of Daphnia magna in the localized area can be used as an index to determine toxicity to pesticides, which is recognized by our image analysis system. The present method is much more time-efficient than the conventional technique based on Japanese Industrial Standards (JIS).
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Key words:@Organophosphorus pesticides, On-line monitoring, Daphnia magna, motion analysis