J. Technology and Education,
Vol.17, No.2, pp.81-86, 2010
研究論文
ジヒドロキシベンズアルデヒド修飾電極によるニコチンアミドアデニンジヌクレオチドの電気化学的酸化
桑原敬司*，阿部嵩也，近藤みずき，下村雅人
長岡技術科学大学 生物系 （〒940-2188 新潟県長岡市上富岡町1603-1）
*
kuwataka@vos.nagaokaut.ac.jp
Electrochemical oxidation of nicotinamide adenine dinucleotide with a dihydroxybenzaldehyde-modified electrode
Takashi KUWAHARA, Syuya ABE, Mizuki KONDO, and Masato SHIMOMURA
Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology
1603-1, Kamitomioka-machi, Nagaoka 940-2188, Japan
(Received November 16, 2010; Accepted December 6, 2010)
Abstract
The electrochemical oxidation of nicotineamide adenine dinucleotide (NADH) was investigated by use of a carbon fiber paper (CFP) electrode modified with 2,5-dihydroxybenzaldehyde (25Q). The modified CFP (25Q/CFP) was prepared by electrochemical polymerization of 25Q in an acetonitrile solution. 25Q/CFP gave a reversible redox wave with a mid-potential (formal potential) of +0.02 V vs. Ag/AgCl in a 0.10 M phosphate buffer (pH 7.0). Electrochemical oxidation of NADH was successfully accomplished with 25Q/CFP, and the oxidation current reached 0.25 mA/cm2 at +0.2 V vs. Ag/AgCl. The onset of oxidation of NADH was found at +0.01 V vs. Ag/AgCl, which corresponds to the mid-potential of 25Q/CFP at pH 7.0. The onset potential was 0.34 V lower than that observed with untreated CFP. The CFP electrodes modified with 3,4-dihydroxybenzaldehyde (34Q) and 2,3-dihydroxybenzaldehyde (23Q) were compared to 25Q/CFP with respect to the oxidation of NADH. The largest NADH oxidation current at +0.2 V was obtained with 23Q/CFP, and the current was 3.5 times as large as that with 25Q/CFP. However, the onset potential observed with 25Q/CFP for the oxidation of NADH was lower than that observed with 34Q/CFP and 23Q/CFP by 0.08 V.
Keywords:
Dihydroxybenzaldehyde, NADH, Quinone, Electrochemical polymerization
