J. Technology and Education, Vol.19, No.2, pp.71-76, 2012

研究論文

チオフェンコポリマー膜を用いて作製した酵素電極の特性

−コポリマー組成が電極特性に及ぼす影響−

出口貴士，近藤みずき，桑原敬司*，下村雅人

長岡技術科学大学 生物系 （〒940-2188 新潟県長岡市上富岡町 1603-1）

*kuwataka@vos.nagaokaut.ac.jp

Properties of enzyme electrodes based on thiophene copolymer films:

effect of the copolymer composition on performance of the electrodes

Takashi DEGUCHI, Mizuki KONDO, Takashi KUWAHARA,

and Masato SHIMOMURA

Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology

1603-1, Kamitomioka-machi, Nagaoka 940-2188, Japan

(Received November 7, 2012; Accepted December 13, 2012)

Abstract

Conducting polymer films were prepared by electrochemical copolymerization of 3-methylthiophene and thiophene-3-acetic acid (T3A). Enzyme electrodes bearing covalently immobilized glucose oxidase (GOx electrodes) were fabricated with the polymer films from the condensation reaction between the enzyme and the carboxyl groups on the films. The electrochemical properties of GOx electrodes were investigated considering the effect of the content of T3A units in the films. The GOx electrodes produced significant oxidation currents in a buffer solution containing glucose and p-benzoquinone. The oxidation current was found to decrease with increasing content of T3A units in the polymer films, which corresponded to a charge in reciprocal of charge transfer resistance (1/R_ct). The reaction between R_ct and the content of T3A units was not affected by the presence of glucose. Both the oxidation current and 1/R_ct decreased by dedoping the polymer films. Thus, the performance of the GOx electrode depended on the conducting properties of the polymer film used.

Key Words: Enzyme electrode, conducting copolymer, polythiophene, glucose oxidase