J. Technology and Education, Vol.20, No.2, pp.59-63(2013)

研究論文

 

細菌のバイオエレクトロカタリシス反応の

微生物分類法への応用

 

高山勝己^*、上島晃智、吉村忠与志

福井工業高等専門学校物質工学科（〒916-8507福井県鯖江市下司町）

*takayama@fukui-nct.ac.jp

 

Application of bioelectrocatalysis as a method of bacterial classification

 

Katsumi TAKAYAMA,* Akinori UEJIMA, Tadayosi YOSHIMURA

Department of Chemistry & Biology Engineering, Fukui National College of Technology

(Geshi-cho, Sabae-shi, Fukui 916-8507, Japan)

 

 (Received July 3, 2013; Accepted August 1, 2013)

 

Abstract

This paper proposes an electrochemical bacterial classification method based on bioelectrocatalysis. Five kinds of quinone compounds (2,3-dimethoxy-5-methyl-1,4-benzoquinone, 1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone, 1,4-benzoquinone, and 2,6-dimethyl-1, 4-benzoquinone)  were used as electron mediators between the anode electrode and the bacterial redox respiratory chain. Escherichia coli (IAM 1264), Staphylococcus aureus (IAM 1011), Lactobacillus casei (IAM 12473), Lactobacillus rhamnosus (ATCC 7469), and Pseudomonas oleovorans (IAM 12410) were selected as typical bacterial cells for the classification test. The data of steady-state current obtained from 25 combinations of quinone and bacteria were analyzed based on principal component analysis. Five bacteria were classified into three groups: Lactobacillus casei (IAM 12473) and Lactobacillus rhamnosus (ATCC 7469), Escherichia coli (IAM 1264) and Pseudomonas oleovorans (IAM 12460), and Staphylococcus aureus (IAM 1011). The Lactobacillus sp. group was closer to the Escherichia coli group than to the Staphylococcus aureus group. These results coincide with classifications based on the 16S rRNA-based sequence homology.

Key Words: Bioelectrocatalysis, Bacterial classification, Principal component analysis, 16S rRNA-based sequence homology analysis