J. Technology and Education, Vol.21, No.1, pp.17-26 (2014)

研究論文

Salacia reticulataのマウス腸管免疫系に対する影響

 

芳野 恭士*¹、間部 涼祐¹、宮本 潤基¹、原　悠樹¹、新井 康介¹、

榊山 達也¹、金高　隆²、古賀 邦正³

¹沼津工業高等専門学校　物質工学科（〒410-8501　沼津市大岡3600）

²株式会社盛光（〒411-0931　静岡県駿東郡長泉町東野50-6）

³東海大学　開発工学部（〒410-0395　沼津市西野317）

*k-yoshino@numazu-ct.ac.jp

 

Effects of Salacia reticulata on the Intestinal Immune System of Mice

 

Kyoji YOSHINO, Ryosuke MANABE, Junki MIYAMOTO, Yuki HARA, Kohsuke ARAI,

Tatsuya SAKAKIYAMA, Takashi KANETAKA, Kunimasa KOGA

¹Numazu College of Technology (3600 Ooka, Numazu, Shizuoka 410-8501, Japan)

² Seiko Co., Ltd (50-6 Higashino, Nagaizumi, Shizuoka 411-0931, Japan)

³ Tokai University (317 Nishino, Numazu, Shizuoka 410-0395, Japan)

 

(Received January 16, 2014; Accepted February 17, 2014)

 

Abstract

In this study, the effects of the stems and leaves of Salacia reticulata on the intestinal immune system of mice were investigated. Oral administration of the stems and leaves of S. reticulata to mice for 28 days suppressed the levels of some cytokines such as interleukin-4 (IL-4) and IL-13, and some antibodies such as immunoglobulin E (IgE) and IgG, in the intestines, spleens, and/or sera of the mice. The intestinal levels of IL-5 were decreased, but the levels of IgA were increased due to elevated levels of IL-6 and IL-10. In the intestines and sera, the levels of IL-12 and interferon–γ (IFN-γ) were increased, and the levels of lipid peroxide and IL-17 were decreased. These results suggest that the administration of the stems and leaves of S. reticulata to mice prompted some immunoregulatory functions in the intestinal and systemic immune systems, such as the suppression of humoral immune responses, the modulation of immune responses by Treg cells, the induction of IgA by activation of the cellular immune response, the suppression of oxidative stress and inflammation, and the suppression of specific inflammatory systems induced by IL-17. In addition, the administration of the leaves of S. reticulata resulted in an increased amount of lactic acid bacteria in the feces of the mice.

Keywords: Salacia reticulata, intestinal immune system, cytokine, antibody