J. Technology and Education, Vol.22, No.1, pp.11-16
(2015)
General Paper
Fluorescent Properties of
Extract from Fired Purple Sea Urchin Shell
Susumu
KOHATA*,
Daiki YAMAGUCHI, Mayu UEMOTO, Reina HIRAOKA,
Keisuke
YOSHINAGA, Yoshisuke FUTAMI and Yuko HAMABE
Department
of Biological and Chemical Systems Engineering, National Institute of
Technology, Kumamoto College, Hirayama-shinmachi
2627, Yatsushiro-city, Kumamoto 866-8501, Japan
(Received
March 23, 2015; Accepted May 11, 2015)
Abstract
The fluorescence properties of an
aqueous solution extracted from fired purple sea urchin shell (PSU) have been
investigated. A pale blue-white fluorescence with a broad spectrum of 420 nm
(peak) and 450 nm (shoulder) was observed by excitation at 370 nm for the
extract from PSU fired at temperatures of 200, 250, 300, 350 and 400 °C. The highest fluorescence intensity was obtained for the extract
from PSU fired at 300 °C. However,
no fluorescence was observed from the extracts of the non-fired shells or from those fired
at 600, 800 and 1000 °C, or from that fired at 300 °C which had been immersed in alkaline solution for 14 days. An oligopeptide with a molecular weight of ca. 910 and some
trace metal ions of Cu and Zn were detected in the extracted aqueous solution
by gel permeation chromatography (GPC), infrared (IR) spectroscopy and
inductively coupled plasma-mass spectrometry (ICP-MS) analyses. These
substances and calcium carbonate are considered to be the source of the
fluorescence.
Key
words:
purple
sea urchin shell, fluorescence, aqueous solution extract, IR, GPC, ICP-MS