J. Technology and Education, Vol.26, No.1, pp.17-22 (2019)

研究論文

シロイヌナズナ種子に対する非対称交流電界の発芽促進効果

高山 勝己^*1，松野 敏英¹，上島 晃智¹，木村 峻¹，田中 良和²，川本 昴³

¹福井工業高等専門学校 物質工学科（〒916-8507 福井県鯖江市下司町）

^*takayama@fukui-nct.ac.jp

²若狭湾エネルギー研究センター 生物資源研究室（〒914-0192 福井県敦賀市長谷64-52-1）

³株式会社ナノ・ブレイン（〒916-0061 福井県鯖江市平井町57-5-13）

 

EFFECT OF ASYMMETRICAL ALTERNATING CURRENT ELECTRIC FIELD

ON GERMINATION OF ARABIDOPSIS THALIANA SEEDS

 

Katsumi TAKAYAMA^*1, Toshihide MATSUNO¹, Akinori UEJIMA¹,

Shun KIMURA¹, Yoshikazu TANAKA² and Subaru KAWAMOTO³

 

¹ Department of Chemistry and Biology Engineering, Fukui National College of Technology

(Geshi-cho, Sabae-shi, Fukui 916-8507, Japan)

² Biology Group, The Wakasa Wan Energy Research Center (Nagatani, Tsuruga-shi, Fukui 914-0192, Japan)

³ Nano-Brain Co., Ltd (Hirai-cho, Sabae-shi, Fukui 916-0061, Japan)

 

(Received January 11, 2019; Accepted February 10, 2019)

 

Abstract

The effect of asymmetrical alternating current electric field (AACEF) on the germination of Arabidopsis thaliana seeds was investigated in this study. AACEF experiments were carried out using a peak value ratio of 3 : 1 (positive voltage : negative voltage), a maximum positive electric field strength of 1,000 V/cm, and a constant frequency of 60 Hz. The seed samples of A. thaliana were exposed to the electric field for 10 days after sowing and were maintained in the light at 25°C for the experimental period. Seed germination was counted daily for 10 days. Total RNA from the seeds was extracted on the first, fifth, and eighth days after sowing. Gene expressions of GA20-oxidase 1 (GA20ox1), GA-INSENSITIVE (GAI), 9-cis-epoxycarotenoid dioxygenase 3 (NCED3), Abscisic acid 8′-hydroxylase 2 (CYP707A2), and 18S ribosomal RNA (used as a housekeeping gene) were analyzed by quantitative real-time PCR. AACEF had no obvious effect on the expression levels of target genes. The germination percentage and the germination rate of the seeds exposed to AACEF were significantly increased from those of the control (without AACEF).

Keywords: Asymmetrical alternating current electric field, Arabidopsis thaliana, Germination