J. Technology and Education, Vol.28, No.1, pp.17-25 (2021)

研究論文

バイオフィルムの定量方法の比較検討

 

甲斐 穂高*1、中根 十愛2、梅川 響1、東浦 芙宇1、平井 信充1

1 鈴鹿工業高等専門学校 生物応用化学科 （〒510-0294 三重県鈴鹿市白子町）

2 鈴鹿工業高等専門学校 専攻科 総合イノベーション工学専攻（〒510-0294 三重県鈴鹿市白子町）

*kai@chem.suzuka-ct.ac.jp

 

Comparison for biofilm quantification methods

 

Hotaka KAI,1 Toa NAKANE,2 Hibiki UMEKAWA,1

Fuu HIGASHIURA,1 and Nobumitsu HIRAI1

1 Department of Chemistry and Biochemistry, National Institute of Technology, Suzuka College,

Shiroko, Suzuka, Mie 510-0294, Japan

2 Advanced Engineering Course of Science and Technology for Innovation, National Institute of Technology,

Suzuka College, Shiroko, Suzuka, Mie 510-0294, Japan

 

(Received April 7, 2021; Accepted May 14, 2021)

 

Abstract
When an environment-improving material that uses steelmaking slag as a raw material is constructed in a sea area, the lack of nutrients in the sea area can be resolved. As a result, marine plants such as algae grow, and the environment of the sea area is improved satisfactorily. In this initial process, a biofilm is formed on the surface of the material. Whether the seaweed grows up depends on the amount of biofilm formed. That is, the quantification of the biofilm formed on the surface of the slag-based environment-improving material is an important factor for the biofilm forming conditions. In this study, the authors investigated a new method of quantifying biofilms formed on the surface of glass substrates and slags by measuring the total organic carbon (TOC) and the bioluminescence of adenosine triphosphate, adenosine diphosphate and adenosine monophosphate (the A3 method).

The greater the amount of biofilm formed on the surface of the glass substrate, the larger the amounts of bioluminescence of ATP and of TOC are detected. From these results, positive correlations were observed between the amount of biofilm and ATPs and the amount of biofilm and TOC. Furthermore, from this viewpoint, the amount of biofilm produced on the slag surface can be evaluated from the amount of bioluminescence of ATPs and the amount of TOC. However, quantifying biofilm by the A3 method is a supplementary position for TOC. From the results, we conclude that it is possible to quantify biofilms by measuring TOC and ATPs.

Key words: Biofilm, Quantification, Slag, TOC, ATPs