J. Technology and Education, Vol.29, No.1, pp.23-27 (2022)

研究論文

オープン型バイポーラ電極を利用した電気化学バイオセンサ

 

栩木 有理沙，近藤 みずき，桑原 敬司*

長岡技術科学大学 生物機能工学専攻（〒940-2188新潟県長岡市上富岡町1603-1）

*kuwataka@vos.nagaokaut.ac.jp

 

Electrochemical biosensor with an open bipolar electrode

Arisa TOCHIGI, Mizuki KONDO, Takashi KUWAHARA*

Department of Bioengineering, Nagaoka University of Technology

(1603-1, Kamitomioka-machi, Nagaoka 940-2188, Japan)

 

(Received April 15, 2022; Accepted May 9, 2022)

 

Abstract

This study proposes a new biosensing method using open bipolar electrodes (OBPEs). OBPEs have the advantage of high flexibility in the configuration of electrochemical cells and electrodes. However, there are few examples of their use in electrochemical biosensors because the electrochemical reaction system is complex and there are fewer electrochemical detection methods to select for comparing closed bipolar electrodes. Therefore, in this study, we aimed to realize a simple biosensor with OBPEs. To achieve the goal, we fabricated a new sensing system with OBPEs and used the system for biomolecule detection. Non-faradic impedance spectroscopy was used for detection. As a result, the sensing system detected the presence of bipolar electrodes as an impedance change. The change rate of impedance was affected by the concentration of electrolytes and the distance between electrodes. Moreover, the sensing system detected proteins immobilized on the bipolar electrodes. These results are significant because they provide new uses for OBPEs and contribute to the development of detection methods for biomolecules.

Keywords: bipolar electrochemistry, biosensor, open bipolar electrodes