J.Technology and Education, Vol.33, No.1, pp.9-16 (2026)
研究論文

ヒトオリゴマンノース糖鎖認識ドメインの酵母細胞表層提示

 

高山 勝己*，大塚 滉生，吹寄 晴輝，松野 敏英

福井工業高等専門学校　物質工学科（〒916-8507福井県鯖江市下司町）

*takayama@fukui-nct.ac.jp

 

Surface display of human mannose binding lectin monomer on the Saccharomyces cerevisiae

 

Katsumi TAKAYAMA*，Koki OTSUKA, Haruki FUKIYOSE and Toshihide MATSUNO

Department of Chemistry & Biology Engineering, National Institute of Technology, Fukui College

(Geshi-cho, Sabae 916-8507, Japan)

 

(Received January 16, 2026; Accepted February 20, 2026)

 

　　In this study, we constructed a plasmid designed to express the carbohydrate-recognition domain (CRD) of human mannose-binding lectin (MBL) on the yeast cell surface using yeast cell-surface engineering technology and successfully displayed the CRD on the cell surface. In the future, yeast cells displaying the CRD on their surfaces are expected to be applied as adsorbents for bacteria such as Salmonella and Escherichia coli, which possess mannose- or N-acetylglucosamine-containing glycans on their cell surfaces.

Keywords: Surface display, Saccharomyces cerevisiae, Mannnose-binding lectin