J. Technology and Education, Vol.20, No.1, pp.7-11 (2013)

研究論文

ポリチオフェン膜にラッカーゼを吸着固定させた

直接電子移動型バイオカソード

 

浅野 健, 近藤みずき, 桑原敬司^＊, 下村雅人

 

長岡技術科学大学　生物系(〒940-2188　新潟県長岡市上富岡町1603-1)

^＊kuwataka@vos.nagaokaut.ac.jp

 

Biocathode composed of polythiophene film and adsorbed laccase involving direct electron transfer

 

Takeshi ASANO, Mizuki KONDO, Takashi KUWAHARA

and Masato SHIMOMURA

 

Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology

(1603-1, Kamitomioka-machi, Nagaoka 940-2188, Japan)

 

(Received November 30, 2012; Accepted January 23, 2013)

 

Abstract

Conducting polymer films were prepared by electrochemical polymerization of thiophene, aniline and pyrrole.  Biocathodes were fabricated by adsorbing laccase on the films in an acetate buffer solution containing laccase.  A bioelectrocatalytic O₂ reduction was investigated with the biocathodes by cyclic voltammetry in 0.05 M acetate buffer solutions (pH 4.5) saturated with O₂ or N₂.  The biocathode fabricated with polythiophene gave the O₂ reduction current based on direct electron transfer (DET) from the polymer to laccase.  The onset potential of the O₂ reduction current was +0.63 V vs. Ag/AgCl.  No O₂ reduction current was obtained using biocathodes fabricated with polyaniline and polypyrrole, whereas adsorbed laccase had O₂-reducing activity.  Thus, DET to active sites of laccase molecules was considered to depend on the properties of the conducting polymer used. 

 

Key words: Biocathode, Direct Electron Transfer, Laccase, Polythiophene