J. Technology and Education, Vol.22, No.2, pp.35-40(2015)

研究論文

サラシア属植物Salacia reticulataのスーパーオキシド

アニオンラジカル捕捉作用

 

芳野 恭士*¹、勝亦 雄太¹、筒井 千尋¹、志賀 早祥¹、芳野 文香¹、片平 俊志¹、

山崎　純¹、一杉 卓矢¹、金高　隆²、古賀 邦正³

¹沼津工業高等専門学校　物質工学科（〒410-8501　沼津市大岡3600）

²株式会社盛光（〒411-0931　静岡県駿東郡長泉町東野50-6）

³東海大学　開発工学部（〒410-0395　沼津市西野317）

*k-yoshino@numazu-ct.ac.jp

 

Superoxide Anion Radical-scavenging Activity of

Salacia Plant, Salacia reticulata

 

Kyoji YOSHINO¹, Yuta KATSUMATA¹, Chihiro TSUTSUI¹, Saaki SHIGA¹,

Ayaka YOSHINO¹, Shunshi KATAHIRA¹, Atsushi YAMAZAKI¹,

Takuya HITOSUGI¹, Takashi KANETAKA², Kunimasa KOGA³

¹Numazu College of Technology (3600 Ooka, Numazu, Shizuoka 410-8501, Japan)

² Seiko Co., Ltd (50-6 Higashino, Nagaizumi, Shizuoka 411-0931, Japan)

³ Tokai University (317 Nishino, Numazu, Shizuoka 410-0395, Japan)

 

(Received November 2, 2015; Accepted November 24, 2015)

 

Superoxide anion radical (O₂^―・)-scavenging activities of aqueous extracts prepared from powdered leaves and stems of a Salacia plant were investigated by the phenazine methosulfate-nicotinamide adenine dinucleotide-nitro blue tetrazolium method. At a final concentration of 0.1 mg/mL, the activities of the extracts of Salacia leaves and stems were higher than those of the extracts of coriander seed, although the activities of Salacia leaves and stems were lower than that of tea leaves. The activity of the Salacia leaf was higher than that of the stem. The mixture of mangiferin, (-)-epicatechin, and (-)-epigallocatechin exhibited the same abilities as compared with the extract of the Salacia leaf in concentrations expected to be included in the extract.  Mangiferin, however, exhibited an inadequate ability as compared with the extract of the Salacia stem at the concentrations expected in 70 mg/mL of the extract. This suggests that polyphenols could be the active O₂^―・-scavenging components of the extracts of Salacia leaves and stems.

Keywords: Salacia reticulata, Antioxidant activity, Superoxide anion radical, Mangiferin, Catechin