J. Technology and Education, Vol.22, No.2, pp.35-40(2015)

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Superoxide Anion Radical-scavenging Activity of

Salacia Plant, Salacia reticulata

 

Kyoji YOSHINO1, Yuta KATSUMATA1, Chihiro TSUTSUI1, Saaki SHIGA1,

Ayaka YOSHINO1, Shunshi KATAHIRA1, Atsushi YAMAZAKI1,

Takuya HITOSUGI1, Takashi KANETAKA2, Kunimasa KOGA3

1Numazu College of Technology (3600 Ooka, Numazu, Shizuoka 410-8501, Japan)

2 Seiko Co., Ltd (50-6 Higashino, Nagaizumi, Shizuoka 411-0931, Japan)

3 Tokai University (317 Nishino, Numazu, Shizuoka 410-0395, Japan)

 

(Received November 2, 2015; Accepted November 24, 2015)

 

Superoxide anion radical (O2\E)-scavenging activities of aqueous extracts prepared from powdered leaves and stems of a Salacia plant were investigated by the phenazine methosulfate-nicotinamide adenine dinucleotide-nitro blue tetrazolium method. At a final concentration of 0.1 mg/mL, the activities of the extracts of Salacia leaves and stems were higher than those of the extracts of coriander seed, although the activities of Salacia leaves and stems were lower than that of tea leaves. The activity of the Salacia leaf was higher than that of the stem. The mixture of mangiferin, (-)-epicatechin, and (-)-epigallocatechin exhibited the same abilities as compared with the extract of the Salacia leaf in concentrations expected to be included in the extract.  Mangiferin, however, exhibited an inadequate ability as compared with the extract of the Salacia stem at the concentrations expected in 70 mg/mL of the extract. This suggests that polyphenols could be the active O2\E-scavenging components of the extracts of Salacia leaves and stems.

Keywords: Salacia reticulata, Antioxidant activity, Superoxide anion radical, Mangiferin, Catechin

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