J. Technology and Education, Vol.21, No.1, pp.1-5 (2025)

研究論文

振とう培養中の大腸菌画像のRGB解析におよぼす

色温度の影響

 

田中 孝国¹*，太田 結月¹，小林 康浩²

¹小山工業高等専門学校　物質工学科 (〒323-0806 小山市中久喜771)

*tanakatakakuni@oyama-ct.ac.jp

²小山工業高等専門学校　電気電子創造工学科 (同上)

 

Effect of color temperature on RGB analysis of images of Escherichia coli in shaking culture

 

Takakuni TANAKA, Yuzuki Ohta and Yasuhiro Kobayashi

National Institute of Technology, Oyama College

(771 Nakakuki, Oyama City, Tochigi 323-0806, JAPAN)

 

(Received December 31, 2024; Accepted February 7, 2025)

 

In this report, we focus on color temperature [K] and investigate the effects of color temperature conditions on captured images. The illuminance conditions were set at 10000, 20000, and 30000 [lx], and the color temperature conditions were set at 2700, 4600, and 6500 [K]. E. coli (strain K-12) was selected as a model bacterium, and liquid shaking culture was conducted using LB medium. Images were taken every 30 minutes from the bottom of the flasks during incubation, and RGB analysis was performed after the images were taken. As a result, it was found that the growth of E. coli can be observed with the change to blue and, for image analysis, it is best to take images at a color temperature of 2700 [K] and an illuminance of 20000 [lx].

Key words: Raspberry Pi / cell concentration / RGB / image analysis / color temperature